nb7516  (Novus Biologicals)

Journal: JCI Insight

Article Title: Vaccine delivery alerts innate immune systems for more immunogenic vaccination

doi: 10.1172/jci.insight.144627

Figure Lengend Snippet: ( A – C ) BALB/c mice were subjected to LPD or ID delivery of OVA or ID delivery of OVA into AFL-treated skin, or IM delivery of OVA in the presence of Alum adjuvant (Alum/IM). Serum anti-OVA IgG ( A ) and subtype IgG1 ( B ) and IgG2a antibody titers ( C ) were measured 3 weeks later. ( D – F ) BALB/c mice were subjected to LPD or ID delivery of 0.15 μg pdm09 vaccine or left nonimmunized (NI). Serum hemagglutination inhibition (HI) titer was measured 3 weeks later ( D ). Mice were challenged with 10 × LD 50 of mouse-adapted pdm09 viruses. Percentage of body weight loss ( E ) and survival ( F ) were monitored daily for 14 days. ( G – I ) C57BL/6 mice were subjected to LPD or ID delivery of pdm09 vaccine or left NI. Serum HI titer was measured 3 weeks later ( G ). Mice were then challenged with 10× LD 50 of mouse-adapted pdm09 viruses. Percentage of body weight loss ( H ) and survival ( I ) were monitored daily for 14 days. ( J – L ) C57BL/6 mice were subjected to LPD or ID delivery of pdm09 vaccine, or IM delivery of pdm09 vaccine in the presence of Alum adjuvant (Alum/IM), or left nonimmunized (NI). Serum HI titer was measured 3 weeks later ( J ). Mice were challenged with 10 × LD 50 of mouse-adapted pdm09 viruses, and percentage of body weight loss ( K ) and survival ( L ) were similarly monitored daily for 14 days. n = 8–9 in A – C , n = 5–10 in D – F , n = 5 in G – I , and n = 8 in J – L . One-way ANOVA with Newman-Keuls multiple-comparison test was used to compare differences between groups ( A – D , G , and J ). Log-rank test with Bonferroni’s correction was used to compare differences of survival between LPD and ID groups ( F , I , and L ). *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: After washing in PBS supplemented with 0.05% Tween 20 (PBST), HRP-conjugated anti-mouse IgG (NA931, GE Healthcare Life Sciences, now Cytiva) or subtype IgG1 (046120, Invitrogen, Thermo Fisher Scientific), IgG2a (NB7516, Novus Biologicals), or IgG2c antibodies (A90-136P, Bethyl Laboratories) were added and incubated at room temperature for 1 hour.

Techniques: Adjuvant, HI Assay, Comparison

Journal: JCI Insight

Article Title: Vaccine delivery alerts innate immune systems for more immunogenic vaccination

doi: 10.1172/jci.insight.144627

Figure Lengend Snippet: WT and MyD88-KO mice were subjected to AFL or sham treatment followed by ID injection of 0.3 μg pdm09 vaccine into AFL- or sham-treated skin. Serum HI titer ( A ), and anti-rHA IgG ( B ) and subtype IgG1 ( C ) and IgG2c antibody titers ( D ) were measured 3 weeks later. n = 9–11. One-tailed Student’s t test was used to compare differences between groups. *, P < 0.05.

Article Snippet: After washing in PBS supplemented with 0.05% Tween 20 (PBST), HRP-conjugated anti-mouse IgG (NA931, GE Healthcare Life Sciences, now Cytiva) or subtype IgG1 (046120, Invitrogen, Thermo Fisher Scientific), IgG2a (NB7516, Novus Biologicals), or IgG2c antibodies (A90-136P, Bethyl Laboratories) were added and incubated at room temperature for 1 hour.

Techniques: Injection, One-tailed Test